Neco Model Questions Vol1 2022 Biology Question 3

Practice practical question 3 from the 2022 Neco Model Questions Vol1 Biology examination. This question covers Nutrition, Carbohydrate analysis, Benedict’s test .

Neco Model Questions Vol1 2022 Biology Practical
Topics: Nutrition Carbohydrate analysis Benedict’s test
Question 3 NECO_MODEL_QUESTIONS_VOL1 • 2022 • BIOLOGY • practical

A student is investigating the carbohydrate content of a locally prepared amala sample using the Benedict’s test.

Question Parts

( a )
Describe the complete procedure for preparing the amala extract and carrying out the Benedict’s test, including at least two safety precautions.
( b )
The following absorbance readings were obtained from three trials after the Benedict’s reaction and dilution to a final volume of 25.0 cm³: Trial 1: 0.312 Trial 2: 0.298 Trial 3: 0.305 A calibration curve prepared with standard glucose solutions is given by: Absorbance = 0.025 × (mg glucose) + 0.005
( ) Calculate the average concentration of glucose (in mg) present in the extract.
( ) Using the dilution factor, determine the % (w/w) carbohydrate in the original 5.00 g sample of amala.
( c )
Identify two possible sources of error in this experiment and suggest a method to minimise each error.

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About This Question

This is Neco Model Questions Vol1 2022 Biology Question 3. It is one of the practical questions from the 2022 Neco Model Questions Vol1 Biology examination.

The question covers Nutrition, Carbohydrate analysis, Benedict’s test .

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