neco 2025 biology | Practical

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Question 1 View Details
You are given a fresh onion (Allium cepa) and a compound microscope. Perform a temporary wet‑mount preparation of an onion epidermal peel and answer the following.
Question Parts
(a)
Outline the step‑by‑step procedure for preparing the wet‑mount slide.
(b)
Identify and label three distinct cell structures that can be seen in the epidermal cells. For each structure, state its principal function.
(c)
State two precautions that must be observed to avoid artefacts during the observation.
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Question 2 View Details
A germinating mung bean (Vigna radiata) root tip is collected and a squash preparation is made for observing mitosis. From five fields of view the following numbers of cells are recorded:
Question Parts
(a)
Calculate the mitotic index for the sample. Show all working.
(b)
Determine the percentage of cells in each phase of mitosis (prophase, metaphase, anaphase, telophase) relative to the total number of mitotic cells. Show your calculations.
(c)
Based on the percentages obtained, which phase appears to be the most prolonged in this sample? Provide a brief biological explanation for this observation.
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Question 3 View Details
A group of students investigated the effect of three different test foods on post‑prandial blood glucose level in healthy volunteers. After an overnight fast, each of five volunteers (V1–V5) consumed 50 g of one test food (Food A, Food B or Food C) on separate days. Blood glucose concentration (mmol/L) was measured with a calibrated glucometer at 0, 30, 60 and 90 minutes after ingestion. The recorded values are shown in the table below. | Volunteer | Food A (0,30,60,90) | Food B (0,30,60,90) | Food C (0,30,60,90) | |-----------|----------------------|----------------------|----------------------| | V1 | 5.0, 6.8, 8.2, 7.5 | 5.1, 6.0, 7.0, 6.5 | 5.0, 5.5, 6.2, 6.0 | | V2 | 4.8, 6.5, 8.0, 7.3 | 4.9, 5.9, 6.9, 6.4 | 4.8, 5.4, 6.1, 5.9 | | V3 | 5.2, 7.0, 8.5, 7.8 | 5.3, 6.2, 7.2, 6.7 | 5.2, 5.6, 6.3, 6.1 | | V4 | 5.1, 6.9, 8.3, 7.6 | 5.0, 6.1, 7.1, 6.6 | 5.1, 5.7, 6.4, 6.2 | | V5 | 5.0, 6.7, 8.1, 7.4 | 5.1, 6.0, 7.0, 6.5 | 5.0, 5.5, 6.2, 6.0 | Using the data above, answer the following questions:
Question Parts
(a)
Calculate the mean blood glucose concentration at 60 minutes for each test food.
(b)
Which food produced the greatest average increase in blood glucose from baseline (0 min) to 60 min? Show your calculation.
(c)
The students claim that the difference between Food A and Food C is statistically significant at the 5 % level. Suggest an appropriate statistical test and briefly justify its use.
(d)
Identify two possible sources of experimental error that could affect the glucose readings and suggest one way to minimise each.
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Question 4 View Details
In a laboratory investigation the rate of respiration of germinating mung bean seeds was measured using a closed‑system manometer. Five fresh mung bean seeds were placed in a 250 mL airtight flask containing 20 mL of distilled water. The flask was connected to a U‑tube manometer filled with water. At the start of the experiment the water level in both arms of the manometer was the same (0 cm). After 10 minutes the water column rose 12 cm in the left arm at 30 °C and 18 cm in the right arm at 40 °C. The temperature of the water bath was maintained constant during each trial. The conversion factor for the set‑up is 1 cm water column = 0.98 mL of CO₂ gas at the experimental temperature and pressure. Answer the following questions:
Question Parts
(a)
Calculate the rate of CO₂ production (in mL min⁻¹) at 30 °C.
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