Neco 2025 Biology Question 33

Two populations of a fish species have evolved different isoforms of the enzyme lactate dehydrogenase (LDH). Their kinetic parameters at 25\(^\circ\)C and 35\(^\circ\)C are given:\n\n\(V_{max}\) (µmol·min^{-1}·mg^{-1}) \(K_{m}\) (µM)\nPopulation X: 120 30\nPopulation X (35\(^\circ\)C): 150 45\nPopulation Y: 100 20\nPopulation Y (35\(^\circ\)C): 130 25\n\nCatalytic efficiency is defined as \(V_{max}/K_{m}\). (i) Which population shows a greater relative increase in catalytic efficiency from 25\(^\circ\)C to 35\(^\circ\)C? (ii) At 35\(^\circ\)C, by what factor is the efficiency of that population greater than the efficiency of the other population? Give the factor to two decimal places.