Neco 2022 Biology Question 23
Practice objective / multiple choice question 23 from the 2022 Neco Biology examination.
An experiment measured the rate of CO\(_2\) production by isolated rat liver mitochondria at four substrate concentrations. The data are:\\ Substrate (mM): 0.5, 1.0, 2.0, 4.0\\ Rate (µmol CO\(_2\) min⁻¹ mg⁻¹ protein): 12, 20, 28, 32.\nAssuming Michaelis-Menten kinetics \(v = \frac{V_{\max}[S]}{K_m+[S]}\), use the two extreme data points (0.5 mM and 4.0 mM) to construct a Lineweaver-Burk plot and estimate \(V_{\max}\) and \(K_m\). Give \(V_{\max}\) to the nearest whole µmol min⁻¹ mg⁻¹ and \(K_m\) to two decimal places (mM).
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About This Question
This is Neco 2022 Biology Question 23. It is one of the objective questions from the 2022 Neco Biology examination.
Difficulty level: Hard .
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