Neco 2021 Biology Question 36

Data from an experiment measuring the activity of enzyme X at various temperatures and pH values are given: at 30°C, pH 7.0 the rate is 90 µmol·min⁻¹; at 40°C, pH 7.0 the rate is 120 µmol·min⁻¹; at 50°C, pH 7.0 the rate is 95 µmol·min⁻¹; at 40°C, pH 6.0 the rate is 80 µmol·min⁻¹. The substrate concentration used was 5 mM and the Michaelis constant of the enzyme is \\(K_{m}=2\\) mM. A competitive inhibitor later reduces the maximum velocity by 30 % without changing \\(K_{m}\\). Determine the temperature at which the enzyme activity is maximal and calculate the inhibited reaction rate (in µmol·min⁻¹) using the Michaelis‑Menten equation \\(v = \\frac{V_{\\max}[S]}{K_{m} + [S]}\\).