Neco Model Questions Vol1 2025 Biology Question 2

Practice practical question 2 from the 2025 Neco Model Questions Vol1 Biology examination. This question covers Cell Division .

Neco Model Questions Vol1 2025 Biology Practical
Topic: Cell Division
Question 2 NECO_MODEL_QUESTIONS_VOL1 • 2025 • BIOLOGY • practical

A fresh root tip of an onion (Allium cepa) is used to prepare a squash slide for observing mitosis. The root tip is 1 cm long, the meristematic zone is identified, and the tissue is fixed, stained with aceto‑orcein and examined under a 40× objective with a 10× ocular. Answer the following:

Question Parts

( a )
Outline the step‑by‑step procedure for preparing a good quality onion root tip squash slide suitable for counting mitotic figures.
( b )
From a random sample of 200 cells observed on the slide, the following distribution was recorded: Interphase – 120 cells Prophase – 30 cells Metaphase – 25 cells Anaphase – 15 cells Telophase – 10 cells (i) Calculate the mitotic index (MI) as a percentage. (ii) Determine the percentage of cells in each of the five phases.
( c )
Explain the biological significance of the mitotic index in assessing the growth activity of a tissue.
( d )
Identify two factors that could lead to an under‑estimation of the mitotic index and suggest how to avoid each factor.

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About This Question

This is Neco Model Questions Vol1 2025 Biology Question 2. It is one of the practical questions from the 2025 Neco Model Questions Vol1 Biology examination.

The question covers Cell Division .

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