Neco Model Questions Vol1 2024 Biology Question 3

Practice practical question 3 from the 2024 Neco Model Questions Vol1 Biology examination. This question covers Nutrition .

Neco Model Questions Vol1 2024 Biology Practical
Topic: Nutrition
Question 3 NECO_MODEL_QUESTIONS_VOL1 • 2024 • BIOLOGY • practical

A student carried out a Benedict’s test to estimate the carbohydrate content of a commercial fruit juice. The procedure was: 1. Weigh 5.00 g of the juice. 2. Dilute with 10 mL distilled water and filter. 3. Pipette 2.00 mL of the filtrate into a test tube, add 2.00 mL of Benedict’s reagent and heat in a boiling water bath for 5 minutes. 4. Cool the mixture, filter the reddish‑brown precipitate, wash with a small amount of cold water and dry to constant mass. The mass of the dried precipitate (Cu₂O) was 0.108 g. The reaction stoichiometry is such that 1.0 g of Cu₂O corresponds to 5.0 g of reducing sugar present in the original sample. The juice label states that it contains 12 % (w/v) carbohydrate (i.e. 12 g per 100 mL). The density of the juice is 1.05 g mL⁻¹. Using the data above, answer the following:

Question Parts

( a )
Calculate the percentage (w/v) of carbohydrate in the juice sample obtained from the experiment. Give your answer to one decimal place.
( b )
State whether the experimental value agrees with the label claim, using an acceptance limit of ±2 % (absolute difference).
( c )
Identify two possible sources of systematic error in the procedure and suggest one method to minimise each error.
( d )
Explain why Benedict’s test measures only reducing sugars and not non‑reducing polysaccharides such as starch.

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This is Neco Model Questions Vol1 2024 Biology Question 3. It is one of the practical questions from the 2024 Neco Model Questions Vol1 Biology examination.

The question covers Nutrition .

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