Neco 2019 Biology Question 3

Practice practical question 3 from the 2019 Neco Biology examination. This question covers Vitamin C determination, Redox titration .

Neco 2019 Biology Practical
Topics: Vitamin C determination Redox titration
Question 3 NECO • 2019 • BIOLOGY • practical

A student carried out a titration to determine the vitamin C (ascorbic acid) content of freshly squeezed orange juice using 2,6‑dichlorophenolindophenol (DCPIP) solution (0.001 M). The procedure was as follows: 1. 50 mL of orange juice was placed in a conical flask. 2. A few drops of starch indicator were added. 3. The juice was titrated against the DCPIP solution until the blue‑black colour disappeared. 4. A blank titration was performed with 50 mL of distilled water under identical conditions. The volumes of DCPIP used are recorded in the table below. | Trial | Volume of DCPIP (mL) | |-------|----------------------| | 1 | 12.3 | | 2 | 12.6 | | 3 | 12.1 | The blank titration gave an average volume of 0.2 mL. The molar mass of vitamin C is 176.12 g mol⁻¹. Answer the following:

Question Parts

( a )
Calculate the average volume of DCPIP required for the orange juice sample after correcting for the blank.
( b )
Using the corrected volume, determine the mass of vitamin C present in the 50 mL orange juice sample (express your answer in milligrams, correct to two decimal places).
( c )
Express the vitamin C concentration as milligrams per 100 mL of juice.
( d )
State the chemical principle on which this titration is based and mention two possible sources of error in the experiment, suggesting one improvement for each.

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About This Question

This is Neco 2019 Biology Question 3. It is one of the practical questions from the 2019 Neco Biology examination.

The question covers Vitamin C determination, Redox titration .

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