Neco 2018 Biology Question 3

Practice practical question 3 from the 2018 Neco Biology examination. This question covers Nutrition, Vitamin C analysis .

Neco 2018 Biology Practical
Topics: Nutrition Vitamin C analysis
Question 3 NECO • 2018 • BIOLOGY • practical

A student is investigating the vitamin C (ascorbic acid) content of fresh orange juice using the DCPIP (2,6-dichlorophenol‑indophenol) titration method. The student prepared a 0.0100 M DCPIP solution and recorded the following data:

Question Parts

( a )
Write the balanced redox equation for the reaction between ascorbic acid (C6H8O6) and DCPIP.
( b )
The titration of 10.0 mL of the orange juice required 12.5 mL of the DCPIP solution. A blank titration (10.0 mL of distilled water) required 1.2 mL of DCPIP. Calculate the concentration of vitamin C in the juice (in mol L⁻¹).
( c )
The weight of the orange sample used to obtain the 10.0 mL juice was 85.0 g. Determine the vitamin C content of the fruit as mg per 100 g.
( d )
State two possible sources of error in this experiment and suggest how each could be minimised.

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About This Question

This is Neco 2018 Biology Question 3. It is one of the practical questions from the 2018 Neco Biology examination.

The question covers Nutrition, Vitamin C analysis .

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