Ijmb Model Questions Vol1 2025 Biology Question 3

Practice practical question 3 from the 2025 Ijmb Model Questions Vol1 Biology examination. This question covers Nutrition .

Ijmb Model Questions Vol1 2025 Biology Practical

Topic: Nutrition

Question 3 IJMB_MODEL_QUESTIONS_VOL1 • 2025 • BIOLOGY • practical

A student is required to determine the carbohydrate content of a powdered food sample using the phenol‑sulfuric acid method. The procedure involves preparing a series of glucose standards, measuring their absorbance at 490 nm, and constructing a calibration curve. The data obtained are:

Question Parts

( a )

Write a concise step‑by‑step procedure for preparing the sample extract and performing the phenol‑sulfuric acid assay.

( b )

Using the standard data below, construct the calibration equation (assume a linear relationship passing through the origin) and determine the concentration of carbohydrate in the sample extract (expressed in mg mL⁻¹).

( c )

Calculate the percentage (w/w) of carbohydrate in the original food sample.

( d )

State two possible sources of experimental error in this assay and suggest a method to minimise each error.

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About This Question

This is Ijmb Model Questions Vol1 2025 Biology Question 3. It is one of the practical questions from the 2025 Ijmb Model Questions Vol1 Biology examination.

The question covers Nutrition .

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