Ijmb Model Questions Vol1 2019 Biology Question 4

Practice practical question 4 from the 2019 Ijmb Model Questions Vol1 Biology examination. This question covers Respiration .

Ijmb Model Questions Vol1 2019 Biology Practical
Topic: Respiration
Question 4 IJMB_MODEL_QUESTIONS_VOL1 • 2019 • BIOLOGY • practical

A group of students study the effect of temperature on the rate of respiration in germinating mung‑bean seeds using a closed respirometer (BOD bottle). Ten seeds (average mass 0.12 g each) are placed in the bottle containing 200 mL of water at a given temperature. The bottle is sealed with a rubber stopper fitted with a graduated gas syringe (capacity 50 mL) that records the decrease in gas volume due to O₂ consumption. The syringe is initially set to 0 mL (fully extended). After 30 min the syringe indicates a contraction of 4.2 mL at 25 °C and 1 atm. The experiment is repeated at 35 °C and the contraction after 30 min is 7.5 mL. Answer the following:

Question Parts

( a )
Calculate the rate of O₂ consumption (in mL O₂ g⁻¹ min⁻¹) at each temperature.
( b )
Assuming a respiratory quotient (RQ) of 1.0 for carbohydrate metabolism, estimate the amount of carbohydrate (as glucose) metabolised per gram of seed in the 30‑minute period at each temperature (expressed in g g⁻¹).
( c )
Using the data, determine the Q₁₀ temperature coefficient for respiration between 25 °C and 35 °C.
( d )
Provide two physiological reasons why respiration rate increases with temperature, and discuss one limitation of the Q₁₀ calculation in this experimental context.

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About This Question

This is Ijmb Model Questions Vol1 2019 Biology Question 4. It is one of the practical questions from the 2019 Ijmb Model Questions Vol1 Biology examination.

The question covers Respiration .

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