Ijmb Model Questions Vol1 2018 Biology Question 4

Practice practical question 4 from the 2018 Ijmb Model Questions Vol1 Biology examination. This question covers Respiration .

Ijmb Model Questions Vol1 2018 Biology Practical
Topic: Respiration
Question 4 IJMB_MODEL_QUESTIONS_VOL1 • 2018 • BIOLOGY • practical

Students investigated the effect of sugar concentration on the rate of cellular respiration in baker's yeast. The procedure was: 1. Prepare three fermentation broths (250 mL each) containing 0 % (control), 2 % (w/v) and 5 % (w/v) glucose respectively. 2. Add 5 g of active dry yeast to each broth, seal the flask with a rubber stopper fitted with a graduated gas‑collecting tube (water displaced), and place the flasks in a water bath at 30 °C. 3. After 30 min, record the volume of CO₂ collected (mL) in each tube. Three independent trials were performed for each concentration. The recorded volumes are: | Glucose concentration | Trial 1 | Trial 2 | Trial 3 | |-----------------------|--------|--------|--------| | 0 % (water control) | 0.2 | 0.1 | 0.15 | | 2 % | 1.8 | 1.9 | 1.7 | | 5 % | 1.2 | 1.1 | 1.3 | Using the data above, answer the following:

Question Parts

( a )
Calculate the mean volume of CO₂ produced for each glucose concentration and the corresponding respiration rate in mL min⁻¹ (use the 30 min incubation period).
( b )
From the calculated rates, state which glucose concentration gives the maximum respiration rate and explain why a higher concentration (5 %) does not produce a higher rate.
( c )
Calculate the percentage inhibition of respiration at 5 % glucose relative to the optimum rate (2 %).
( d )
Suggest one procedural improvement that could reduce experimental error in measuring CO₂ volume.

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This is Ijmb Model Questions Vol1 2018 Biology Question 4. It is one of the practical questions from the 2018 Ijmb Model Questions Vol1 Biology examination.

The question covers Respiration .

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